CHAPTER 7
INTERACTION OF SWP WITH METALS AND BASIC PROTEINS
Introduction
Metal binding studies
Function
Binding properties
Materials
Method
Method-A
Method-B
Interaction of SWP with Lysozyme and Clupeine
Mode of action of Lysozyme
Clupeine
Materials and Methods
Visual Assessment of SWP:clupeine and SWP:lysozyme mixtures
Materials
Sample preparation
Method
Dynamic Mechanical Testing
Materials
Method
Phase contrast microscopy
Materials
Method
Haematoxylin and Eosin Staining
Materials
Method
Differential Scanning Calorimetry(DSC)
Materials
Method
Lysozyme turbidity assay
Materials
Sample preparation
Method
RESULTS
Metal binding studies
Interaction of SWP, lysozyme and clupeine
Visual Assessment of SWP:clupeine and SWP:lysozyme mixtures
Dynamic Mechanical Testing
Differential Scanning Calorimetry(DSC)
Phase contrast microscopy
Haematoxylin and Eosin Staining
Lysozyme turbidity assay
DISCUSSION
Metal binding studies
Interaction of SWP with lysozyme and clupeine
CONCLUSIONS