CHAPTER 5
INTERACTION OF SWP WITH BOVINE SERUM ALBUMIN (BSA)
Introduction
Bovine serum albumin (BSA)
Structure of BSA
Amino acid composition
Primary, secondary and tertiary structure
Disulphide bonds
Physical-Chemical Properties
Viscosity
Effects of pH
Effect of Heat
Functional properties of BSA
Foaming
Gelation properties of BSA
Ligand-binding
MATERIALS AND METHODS
BSA Unfolding
Introduction
Materials
Method
Dynamic Mechanical Testing
Materials
Method
Phase separation
Materials
Method
RESULTS
BSA unfolding
Dynamic Mechanical Testing
Phase Separation Studies
DISCUSSION
BSA structure
Effect of Guanidine HCl
Effect of Heating
CONCLUSIONS